Linear amplification of short nucleic acids
US9394557B2 · kind B2 · utility
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20Claims
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Key dates
| Filing date | Dec 20, 2013 |
| Grant date | Jul 19, 2016 |
| Priority date | — |
| Expiry date | May 14, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6853
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present teachings provide novel methods for amplifying short nucleic acids. In some embodiments, the present teachings provide novel methods for linearly amplifying a collection of micro RNAs by using temperature cycling during a reverse transcription reaction. The cycling can comprise at least 20 cycles of an annealing temperature segment of 10° C.-30° C., and a denaturation temperature segment of 35° C.-60° C. In some embodiments, the temperature cycled reaction can comprise an osmolyte.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.