Patent · US Active

Method for obtaining immunoglobulin encoding nucleic acid

US9399670B2 · kind B2 · utility

0Cited by

4References

8Claims

0Family size

Assignee

Hoffmann-La Roche Inc. · US

Inventors

Hans-Willi Krell · Penzberg, DE
Alexander Lifke · Penzberg, DE
Valeria Lifke · Penzberg, DE
Kairat Madin · Penzberg, DE
Christian Weilke · Penzberg, DE

Key dates

Filing date	Feb 18, 2010
Grant date	Jul 26, 2016
Priority date	—
Expiry date	Jan 23, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2600/16
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The current invention is directed to a method for obtaining a nucleic acid encoding an immunoglobulin variable domain from a single cell comprising the following steps: —performing a first polymerase chain reaction with three to six 5′-primer and one 3′-primer, performing with the product of the first polymerase chain reaction a second polymerase chain reaction with thirteen to sixteen 5′-primer and one 3′-primer, whereby the distance of the binding locations of the primer employed in the second polymerase chain reaction is reduced compared to the first polymerase chain reaction.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.