Patent · US Active

Method for preventing high molecular weight products during amplification

US9447476B2 · kind B2 · utility

1Cited by

8References

17Claims

0Family size

Assignee

Roche Molecular Systems, Inc. · US

Inventors

Reiner Babiel · Eberfing, DE
Frank Bergmann · Iffeldorf, DE
Dorothea Sizmann · Iffeldorf, DE

Key dates

Filing date	Oct 16, 2013
Grant date	Sep 20, 2016
Priority date	—
Expiry date	Oct 16, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/686
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention is directed to improved methods for amplifying and detecting a nucleic acid target that may be present in a biological sample comprising a primer pair for generating an amplicon, wherein at least one primer is modified at the 5′ terminus with a polyN sequence being non-complementary to the target sequence, and a detectable probe specific for said amplicon or a DNA binding dye. The formation of high molecular weight products during amplification is prevented or partly or completely suppressed. The invention further provides reaction mixtures and kits comprising said primers for preventing or suppressing the formation of high molecular weight products during amplification and detection of the nucleic acid target.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.