Multiplex compositions and methods for quantification of human nuclear DNA and human male DNA and detection of PCR inhibitors
US9481909B2 · kind B2 · utility
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7Claims
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Key dates
| Filing date | Jun 19, 2015 |
| Grant date | Nov 1, 2016 |
| Priority date | — |
| Expiry date | Jun 19, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/166
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.