Detection and measurement of tissue-infiltrating lymphocytes
US9499865B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 29, 2012 |
| Grant date | Nov 22, 2016 |
| Priority date | — |
| Expiry date | Nov 29, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5094
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention is drawn to methods for measuring numbers, levels, and/or ratios of cells, such as lymphocytes, infiltrated into a solid tissue, such as a tumor or a tissue affected by an autoimmune disease, and to methods for making patient prognoses based on such measurements. In one aspect, methods of the invention comprise sorting lymphocytes from an accessible tissue, such as peripheral blood, into functional subsets, such as cytotoxic T cells and regulatory T cells, and generating clonotype profiles of each subset. An inaccessible disease-affected tissue is sampled and one or more clonotype profiles are generated. From the latter clonotype profiles, levels lymphocytes in each of the functional subsets are determined in the disease-affected tissue by their clonotypes, which are identified from lymphocytes sorted into subsets from the accessible tissue.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.