Rapid adaptive optical microscopy over large multicellular volumes
US9500846B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 17, 2015 |
| Grant date | Nov 22, 2016 |
| Priority date | — |
| Expiry date | Mar 19, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/0675
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Excitation light is focused to a focus within a sample and the focus is scanned within a volume in the sample with scanning optical elements. Signal light emitted from the focus is de-scanned, with the one or more scanning optical elements, onto a wavefront sensor as the focus is scanned within the volume. Based on the descanned signal light, an average aberration created by the volume of the sample of a wavefront of the excitation light is determined. A wavefront of the excitation light is corrected by an amount according to the determined average aberration while the focus is scanned within the volume, the signal light is imaged onto a photosensitive detector as the focus is scanned within the volume, and a wavefront of the imaged signal light is corrected by an amount according to the determined average aberration while the focus is scanned. These steps can be repeated for a plurality of different volumes in the sample, and an image of the sample can be generated based on the detected signal light from scanned foci within the different volumes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.