Method of sequence determination using sequence tags

US9506119B2 · kind B2 · utility

Assignee

• Adaptive Biotechnologies Corporation · US

Inventors

• Malek Faham · Pacifica, US
• Martin Moorhead · San Mateo, US
• Thomas Willis · San Francisco, US
• Jianbiao Zheng · Fremont, US

Key dates

• Filing date: Mar 15, 2013
• Grant date: Nov 29, 2016
• Expiry date: Jan 24, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/158
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention is directed to the use of sequence tags to improve sequence determination of amplicons of related sequences, particularly large and complex amplicons, such as those comprising recombined nucleic acids encoding immune receptor molecules. In one aspect, sequence reads having the same sequence tags are aligned after which final base calls are determined from a (possibly weighted) average base call from sequence read base calls at each position. Similarly, in another aspect, sequence reads comprising series of incorporation signals are aligned by common sequence tags and base calls in homopolymer regions are made as a function incorporation signal values at each “flow” position.