Thermostable type-A DNA polymerase mutants with increased polymerization rate and resistance to inhibitors
US9523085B2 · kind B2 · utility
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18Claims
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Key dates
| Filing date | Aug 2, 2010 |
| Grant date | Dec 20, 2016 |
| Priority date | — |
| Expiry date | Jun 8, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1252
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides mutants of DNA polymerases having an increased rate of incorporation of nucleotides into nucleic acids undergoing polymerization and having an enhanced resistance to inhibitors of DNA polymerase activity. The mutant polymerases are well suited for fast PCR applications, for PCR amplification of targets in samples that contain inhibitors of wild-type polymerases, and for fast PCR amplification of samples containing DNA polymerase inhibitors. In exemplary embodiments, the mutants are mutants of Taq DNA polymerase.
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