DNA sequencing methods and detectors and systems for carrying out the same

US9528152B2 · kind B2 · utility

Assignee

• Life Technologies Corporation · US

Inventors

• Hongye Sun · Karlsruhe, DE
• Mark F. Oldham · Los Gatos, US
• John O'Neill · Coral Springs, US
• Charles R. Connell · Redwood City, US
• Umberto Ulmanella · Foster City, US
• Aldrich N. K. Lau · Palo Alto, US
• Theo Kotseroglou · San Diego, US
• Kenneth J. Livak · San Jose, US

Key dates

• Filing date: Dec 2, 2014
• Grant date: Dec 27, 2016
• Expiry date: Dec 2, 2034

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/143333
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures. Surface-modified nanopores are provided as are methods of making them. In some embodiments, surfaced-modified nanopores for slowing the translocation of single stranded DNA (ssDNA) through the nanopore are provided,…