MGMT-based method for obtaining high yields of recombinant protein expression
US9546380B2 · kind B2 · utility
3Cited by
4References
22Claims
0Family size
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Key dates
| Filing date | Jul 28, 2015 |
| Grant date | Jan 17, 2017 |
| Priority date | — |
| Expiry date | Jul 28, 2035 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a novel enhancer of protein production in host cells. It discloses a vector for expressing recombinant proteins in these cells, comprising a nucleotide sequence encoding a) a secretion peptidic signal, b) a 6-methylguanine-DNA-methyltransferase enzyme (MGMT, EC 2.1.1.63), a mutant or a catalytic domain thereof, and c) a recombinant protein. Said MGMT enzyme is preferably the so-called SNAP protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.