Patent · US Active

IGF-I poly (ethylene glycol) conjugates

US9587006B2 · kind B2 · utility

0Cited by

3References

3Claims

0Family size

Assignee

Hoffmann-La Roche Inc. · US

Inventors

Friederike Hesse · Munich, DE
Eva Hoess · Starnberg, DE
Stephanie Mueller · Neuruppin, DE
Eva Maria Trost-Gross · Wolfratshausen, DE

Key dates

Filing date	Feb 4, 2011
Grant date	Mar 7, 2017
Priority date	—
Expiry date	Feb 4, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12P21/06
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

Herein is reported a method for producing of a polypeptide conjugated to one poly (ethylene glycol) comprising a) providing a nucleic acid encoding an expression construct comprising in 5′ to 3′ direction a nucleic acid encoding a polypeptide, and a nucleic acid encoding a trypsin site of SEQ ID NO: 01, b) expressing the nucleic acid of a) in a cell and recovering the expression construct from the cell and/or the cultivation medium, c) providing a target peptide with an amino acid sequence of SEQ ID NO: 02 covalently conjugated to a poly (ethylene glycol) at the C-terminal lysine residue, d) incubating the expression construct and the target peptide with the trypsin mutant D189K, K60E, N143H, E151H, and e) recovering and thereby producing the polypeptide conjugated to one poly (ethylene glycol) from the incubation mixture.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.