Structurally designed shRNAs
US9624494B2 · kind B2 · utility
10Cited by
9References
19Claims
0Family size
Assignee
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Key dates
| Filing date | Feb 24, 2015 |
| Grant date | Apr 18, 2017 |
| Priority date | — |
| Expiry date | Feb 24, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2320/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is an improved design of shRNA based on structural mimics of miR-451 precursors. These miR-451 shRNA mimics are channeled through a novel small RNA biogenesis pathway, require AGO2 catalysis and are processed by Drosha but are independent of DICER processing. This miRNA pathway feeds active elements only into Ago2 because of its unique catalytic activity. These data demonstrate that this newly identified small RNA biogenesis pathway can be exploited in vivo to produce active molecules.
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