Patent · US Active

Method for the operation of a laser scanning microscope

US9671341B2 · kind B2 · utility

0Cited by
5References
8Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJul 9, 2015
Grant dateJun 6, 2017
Priority date
Expiry dateJul 9, 2035

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N2201/0675
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Method for the operation of a laser scanning microscope. The microscope includes an illumination beam path in which at least one illumination light source is arranged, a detection beam path in which at least one photomultiplier (PMT) is incorporated as detector, and a control unit for controlling fluorescence experiments. A sample is alternately illuminated at high intensity via the control unit, and the fluorescence decay behavior of sample points and/or sample regions is subsequently detected. The PMT is switched on and off depending on the illumination mode by the control unit via a switch directly in the high-voltage supply of the PMT.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.