Method for the operation of a laser scanning microscope
US9671341B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 9, 2015 |
| Grant date | Jun 6, 2017 |
| Priority date | — |
| Expiry date | Jul 9, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/0675
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Method for the operation of a laser scanning microscope. The microscope includes an illumination beam path in which at least one illumination light source is arranged, a detection beam path in which at least one photomultiplier (PMT) is incorporated as detector, and a control unit for controlling fluorescence experiments. A sample is alternately illuminated at high intensity via the control unit, and the fluorescence decay behavior of sample points and/or sample regions is subsequently detected. The PMT is switched on and off depending on the illumination mode by the control unit via a switch directly in the high-voltage supply of the PMT.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.