Detecting single nucleotide polymorphism using overlapping hydrolysis probes
US9689026B2 · kind B2 · utility
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Key dates
| Filing date | Aug 9, 2016 |
| Grant date | Jun 27, 2017 |
| Priority date | — |
| Expiry date | Aug 9, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for the rapid detection of the presence or absence of a SNP in a target nucleic acid in a sample are described. The methods can include performing an amplifying step, a hybridizing step utilizing a double stranded probe with two overlapping SNP specific hydrolysis probe sequences where one of the probe sequences can include a hairpin structure toward the 3′ end, and a detecting step. Furthermore, the double stranded SNP specific hydrolysis probes along with kits are provided that are designed for the detection of a SNP in a target nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.