Microscope and method for SPIM microscopy

US9715095B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Jörg Ritter · Baden-Baden, DE
• Helmut Lippert · Jena, DE
• Tobias Kaufhold · Jena, DE

Key dates

• Filing date: Oct 6, 2012
• Grant date: Jul 25, 2017
• Expiry date: Jan 28, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B21/367
• WIPO field: Optics
• WIPO sector: Instruments

Abstract

A method for SPIM microscopy, wherein the sample is moved continuously, and a plurality of images are taken at time intervals by means of a detection arrangement during the movement. The image capture duration or exposure time is dimensioned such that the movement path of the sample lies within a predetermined resolution range of the detection objective. The speed of the sample movement is determined and set by the image capture duration or exposure time and/or the distortion of the point spread function generated by the sample movement of the sample. The image blur is corrected computationally by the respective image capture duration and the movement speed. A sharp image is generated in this way. The actual optical section thickness of the light sheet is determined from the light sheet thickness, and the movement speed is determined therefrom and from user settings.