Patent · US Active

Method for embedding a biological sample in a transparent matrix for analysis using single plane illumination microscopy

US9733160B2 · kind B2 · utility

1Cited by

0References

2Claims

0Family size

Assignee

Carl Zeiss Microscopy GmbH · DE

Inventors

Wibke Hellmich · Jena, DE
Benno Radt · Jena, DE
Helmut Lippert · Jena, DE
Olaf Selchow · Jena, DE
Uwe Wolf · Magdala, DE
Juergen Haese · Gleichen, DE

Key dates

Filing date	Dec 18, 2013
Grant date	Aug 15, 2017
Priority date	—
Expiry date	Feb 13, 2034

Classification

Technology area (CPC G)Physics
CPC primaryG02B21/34
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The invention is directed to method for positioning and aligning a preferably biological sample in the detection area of the objective of a microscope arrangement. According to the invention, the method mentioned above has the following method steps: a sample is introduced into a transparent medium, preferably agarose gel, which is initially liquid; the medium is changed from the liquid state to the solid state, wherein the sample is fixated within the medium, but the transparency of the medium is retained; the solidified medium is positioned in the microscope arrangement in such a way that the sample enclosed therein is situated in the detection area of the objective. Further, a device is proposed for positioning and aligning a preferably biological sample in the detection area of the objective of a microscope arrangement.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.