In vitro method for estimating in vivo protein digestibility
US9738920B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jan 16, 2015 |
| Grant date | Aug 22, 2017 |
| Priority date | — |
| Expiry date | Jan 16, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6806
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An analysis technique can be performed to quantify the digestible protein content of a protein-containing sample outside the body of a living organism. Traditionally, protein digestibility is evaluated in vivo, for example using a rat subject to measure protein digestibility after being fed the protein-containing sample. In some examples, an in vitro technique involves enzymatically digesting the protein-containing sample to simulate digestion that would occur inside a mammalian body. The sample can then be optically analyzed to measure the amount of reactive amine present in the sample, which can provide an indication of the amount of amino acid released during digestion. In some examples, the measured reactive amine value is adjusted to account for the stronger and/or weaker optical response of certain amino acids due to their relative reactivity with an optical tagging agent. Thereafter, an in vivo protein digestibility value can be calculated based on adjusted amine concentration.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.