Paired end bead amplification and high throughput sequencing
US9738930B2 · kind B2 · utility
2Cited by
14References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 27, 2012 |
| Grant date | Aug 22, 2017 |
| Priority date | — |
| Expiry date | Mar 26, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B50/14
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a paired end sequencing method that enables the sequencing of unique read pairs by co-localizing both 5′ ends on a single emulsion polymerase chain reaction bead. The method may use a customized forked adaptor primer pair that is compatible with massively parallel sequencing techniques. The compositions and methods disclosed herein contemplate sequencing complex genomes, amplified genomic regions, as well as detecting chromosomal structural rearrangements.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.