Compositions relating to a mutant Clostridium difficile toxin and methods thereof

US9745354B2 · kind B2 · utility

Assignee

• Wyeth, LLC · US

Inventors

• Mark Edward Ruppen · Garnerville, US
• Justin Keith Moran · Valley Cottage, US
• Kathrin Ute Jansen · Doylestown, US
• Annaliesa Sybil Anderson · Upper Saddle River, US
• Robert G. K. Donald · South Orange, US
• Narender Kumar Kalyan · Ridgewood, US
• Maninder K. Sidhu · New City, US
• Michael James Flint · Decatur, US

Key dates

• Filing date: Oct 31, 2014
• Grant date: Aug 29, 2017
• Expiry date: Oct 31, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/92
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

In one aspect, the invention relates to an immunogenic composition that includes a mutant Clostridium difficile toxin A and/or a mutant Clostridium difficile toxin B. Each mutant toxin includes a glucosyltransferase domain having at least one mutation and a cysteine protease domain having at least one mutation, relative to the corresponding wild-type C. difficile toxin. The mutant toxins may further include at least one amino acid that is chemically crosslinked. In another aspect, the invention relates to antibodies or binding fragments thereof that binds to said immunogenic compositions. In further aspects, the invention relates to isolated nucleotide sequences that encode any of the foregoing, and methods of use of any of the foregoing compositions.