Patent · US Active

Methods and compositions for targeted cleavage and recombination

US9782437B2 · kind B2 · utility

9Cited by
33References
8Claims
0Family size

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Key dates

Filing dateJul 31, 2014
Grant dateOct 10, 2017
Priority date—
Expiry dateJul 31, 2034

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2800/80
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.