Patent · US Active

Method for separation of monomeric polypeptides from aggregated polypeptides

US9783570B2 · kind B2 · utility

2Cited by

5References

3Claims

0Family size

Assignee

Hoffmann-La Roche Inc. · US

Inventors

Monika Baehner · Munich, DE
Adelbert Grossmann · Murnau am Staffelsee, DE
Stefan Hepbildikler · Munich, DE

Key dates

Filing date	Jun 28, 2012
Grant date	Oct 10, 2017
Priority date	—
Expiry date	Jun 28, 2032

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2319/30
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Herein is reported a method for obtaining a polypeptide in monomeric form comprising the steps of a) providing a solution comprising the polypeptide m monomeric form and m aggregated form, wherein the ratio of monomeric to aggregated form is 4:1 or less as determined by size exclusion chromatography, b) performing a mixed-mode chromatography in bind-and-elute mode, or a hydrophobic interaction chromatography in flow-through mode, or a size-exclusion chromatography, and c) performing a weak cation exchange chromatography in bind-and-elute mode or flow-through mode, and thereby obtaining the polypeptide m monomeric form.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.