Method for the detection and quantitation of biomarkers

US9903868B2 · kind B2 · utility

Assignee

• The United States of America, as represented by the Secretary, Department of Health and Human Services · US

Inventors

• Xiaoyuan Chen · Acton, US
• Dingbin Liu · Tianjin, CN

Key dates

• Filing date: May 18, 2015
• Grant date: Feb 27, 2018
• Expiry date: May 18, 2035

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/587
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention provides a method for detecting the presence or absence of a biomarker in a biological sample at a very low concentration comprising the steps of (a) contacting the biological sample with a capture binding sequence immobilized on a surface, (b) providing a conjugate comprising a detection binding sequence-glucose oxidase, (c) contacting the surface with the detection binding sequence-glucose oxidase conjugate, (d) separating any unbound detection binding sequence-glucose oxidase conjugate from the surface, (e) incubating the resulting surface with a glucose solution and a mixture comprising gold nanoparticles and a gold salt, wherein the gold nanoparticles have an initial particle size of about 5 nm, and (f) observing any change in color of the mixture. The invention also provides a method for diagnosing the presence of a prostate cancer biomarker in a subject and a kit for detecting or quantifying a biomarker in a biological sample.