Method of detection of cAMP and cGMP via a fluorophore and dequencher complex
US9915650B2 · kind B2 · utility
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4Claims
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Key dates
| Filing date | Dec 13, 2007 |
| Grant date | Mar 13, 2018 |
| Priority date | — |
| Expiry date | Sep 4, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5735
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to an in vitro method for detecting cAMP or cGMP comprising a) contacting a mixture with a complex of a tracer and a dequencher, wherein the tracer is a fluorophore covalently linked to a cAMP quencher, and b) measuring the change in fluorescence. Furthermore, the present invention relates to the use of said method for determining the cAMP or cGMP concentration in the mixture, for determining the activity of a receptor wherein the signal transduction of the receptor comprises cAMP or cGMP and for screening of a ligand for such a receptor.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.