Patent · US Active

Specific targeting of RNA expanded repeat sequences

US9933419B2 · kind B2 · utility

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7Claims
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Key dates

Filing dateFeb 8, 2017
Grant dateApr 3, 2018
Priority date
Expiry dateFeb 8, 2037

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N2500/10
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

The present invention provides small molecule compounds that can form covalent adducts with specific sequences of RNA, such as the hairpin loop r(CUG)exp sequence which is a cause of myotonic dystrophy type 1 (DM1), or the r(CGG)exp sequence which is a cause of fragile X-associated tremor/ataxia syndrome (FXTAS); to methods of making the small molecule compounds; and to methods of using the small molecular compounds in the treatment of DM1 or of FXTAS in patients afflicted therewith. The invention further provides a method for identifying an RNA target of a small molecule drug in vivo, using a small molecule drug conjugated to an RNA-reactive crosslinker group and a reporter group, contacting a cell or nucleic acid extract with the small molecule drug conjugate, then separating RNA targets crosslinked to the small molecule drug conjugate by interaction of the affinity group with a complementary affinity group.

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