Expression of eukaryotic polypeptides in chloroplasts
USRE44266E1 · kind E1 · reissue
Assignee
Inventor
Key dates
| Filing date | Jan 16, 1998 |
| Grant date | Jun 4, 2013 |
| Priority date | — |
| Expiry date | Jan 16, 2018 |
Classification
- Technology area (CPC —)General
Abstract
The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.