Optical arrangement for the production of a light-sheet

USRE45575E1 · kind E1 · reissue

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Helmut Lippert · Jena, DE
• Matthias Wald · Jena, DE
• Benno Radt · Jena, DE

Key dates

• Filing date: Jul 1, 2013
• Grant date: Jun 23, 2015
• Expiry date: Jul 1, 2033

Classification

• Technology area (CPC —): General

Abstract

The invention is directed to an optical arrangement with a light source for emitting a light bundle and with optical elements for transforming this light bundle into the shape of a light sheet, particularly suitable for illuminating individual planes of a three-dimensional specimen in selective plane illumination microscopy (SPIM). According to the invention, means are provided for varying the cross section of the light sheet, for varying the length of the light sheet and/or for influencing the direction in which individual beam components extending within the light sheet are directed to the specimen substance. This makes it possible to adapt the geometry of the light sheet to the illumination requirements for observing one and the same specimen plane with a plurality of different objectives and, if required, to reduce shadows occurring within the observed specimen plane as a result of the illumination.