Compositions relating to a mutant Clostridium difficile toxin and methods thereof

USRE46518E1 · kind E1 · reissue

Assignee

• Wyeth, LLC · US

Inventors

• Annaliesa Sybil Anderson · Upper Saddle River, US
• Maninder K. Sidhu · New City, US
• Robert G. K. Donald · South Orange, US
• Kathrin Ute Jansen · Doylestown, US
• Narender Kumar Kalyan · Ridgewood, US
• Justin Keith Moran · Valley Cottage, US
• Mark Edward Ruppen · Garnerville, US
• Michael James Flint · Decatur, US

Key dates

• Filing date: Dec 1, 2016
• Grant date: Aug 22, 2017
• Expiry date: Dec 1, 2036

Classification

• Technology area (CPC —): General

Abstract

In one aspect, the invention relates to an immunogenic composition that includes a mutant Clostridium difficile toxin A and/or a mutant Clostridium difficile toxin B. Each mutant toxin includes a glucosyltransferase domain having at least one mutation and a cysteine protease domain having at least one mutation, relative to the corresponding wild-type C. difficile toxin. The mutant toxins may further include at least one amino acid that is chemically crosslinked. In another aspect, the invention relates to antibodies or binding fragments thereof that binds to said immunogenic compositions. In further aspects, the invention relates to isolated nucleotide sequences that encode any of the foregoing, and methods of use of any of the foregoing compositions.