Nucleic acid terminators incorporating a cationic moiety and methods for their use

USRE46683E1 · kind E1 · reissue

Assignee

• Applied Biosystems, LLC · US

Inventors

• Barnett Rosenblum · San Jose, US
• Steven M. Menchen · Fremont, US
• Shaheer H. Khan · Foster City, US
• Paul Kenney · Sunnyvale, US

Key dates

• Filing date: Sep 30, 2014
• Grant date: Jan 23, 2018
• Expiry date: Sep 30, 2034

Classification

• Technology area (CPC —): General

Abstract

Disclosed are methods and kits applicable to sequencing methods, such as Sanger dideoxy sequencing methods. The methods and kits disclosed utilize a cationically charged nucleic acid terminator in combination with a discriminatory polymerase.