Transposase-random priming DNA sample preparation
USRE49207E1 · kind E1 · reissue
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| Filing date | Nov 8, 2019 |
| Grant date | Sep 13, 2022 |
| Priority date | — |
| Expiry date | Nov 8, 2039 |
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- Technology area (CPC —)General
Abstract
Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided.
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