DNA polymerases and related methods

US10035993B2 · kind B2 · utility

Assignee

• Roche Molecular Systems, Inc. · US

Inventors

• Keith Bauer · Los Altos, US
• Ellen H. Fiss · Albany, US
• David H. Gelfand · Oakland, US
• Edward S. Smith · San Francisco, US
• Shawn Suko · El Sobrante, US
• Thomas W. Myers · Dublin, US

Key dates

• Filing date: Jul 6, 2017
• Grant date: Jul 31, 2018
• Expiry date: Jul 6, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P19/34
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed are mutant DNA polymerases having improved extension rates relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.