Device for optical examination of a specimen, method for examining a specimen and method for transferring a device into an operation-ready state

US10345570B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Wolfgang Singer · Aalen, DE
• Aksel Goehnermeier · Aalen, DE
• Guenter Rudolph · Jena, DE
• Johannes Ruoff · Aalen, DE
• Christian Dietrich · Jena, DE
• Rong Dong · East Brunswick Township, US

Key dates

• Filing date: Oct 28, 2016
• Grant date: Jul 9, 2019
• Expiry date: May 27, 2037

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/0335
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a device for optical examination of a specimen with a cryo-immersion objective, having a stative, to which the cryo-immersion objective is fixed, wherein the cryo-immersion objective has a plurality of optical components, in particular lenses, and wherein the cryo-immersion objective has an optical front component which is in contact during operation with a coolable immersion liquid, having a specimen carrier for a specimen to be examined, having means for providing a cooled immersion liquid between the optical front component and the specimen to be examined on or against the specimen carrier. The device is characterized in that insulating means are present for interrupting a heat transition between the stative and the optical front component. The invention also relates to a method for examining a specimen, wherein with a device according to the invention a plurality of microscopic images are recorded, wherein for each of the individual images a different offset between a main housing and a separate housing is set. Finally the invention relates to a method for transferring a device according to the invention into an operation-ready state, wherein the compon…