Method of aligning a laser-scanning fluorescence microscope and laser-scanning fluorescence microscope having an automatic aligning system

US11131630B2 · kind B2 · utility

Assignee

• ABBERIOR INSTRUMENTS GMBH · DE

Inventors

• Joern Heine · Witzenhausen, DE
• Matthias Reuss · Sundbyberg, SE
• Benjamin Harke · Oettingen, DE
• Lars Kastrup · Göttingen, DE

Key dates

• Filing date: Feb 26, 2019
• Grant date: Sep 28, 2021
• Expiry date: Jul 2, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B21/008
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

For setting a laser-scanning fluorescence microscope to a correct alignment in which an intensity maximum of excitation light and an intensity minimum of fluorescence inhibition light coincide in a focal area of an objective lens, a structure in a sample marked with a fluorescent dye is scanned with the intensity maximum of the excitation light to generate first and second pictures of the sample, the first picture corresponding to a higher and the second picture corresponding to a lower intensity of the fluorescence inhibition light. A spatial offset of a first image of the structure in the first picture with regard to a second image of the structure in the second picture is calculated; and the intensity maximum of the excitation light is shifted with regard to the intensity minimum of the fluorescence inhibition light in the direction of the offset calculated to set the microscope to the correct alignment.