Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction

US11214797B2 · kind B2 · utility

Assignees

• The Broad Institute, Inc. · US
• Massachusetts Institute of Technology · US
• The Regents of the University of California · US

Inventors

• Aviv Regev · Cambridge, US
• Brittany Adamson · San Francisco, US
• Brian P. Cleary · Somerville, US
• Le Cong · Cambridge, US
• Atray Dixit · Cambridge, US
• Jellert Gaublomme · Cambridge, US
• Eric S. Lander · Cambridge, US
• Thomas Norman · Oklahoma City, US
• Oren Parnas · Jerusalem, IL
• Orit Rozenblatt-Rosen · Cambridge, US
• Alexander K. Shalek · Cambridge, US
• Jonathan Weissman · San Francisco, US

Key dates

• Filing date: Apr 27, 2018
• Grant date: Jan 4, 2022
• Expiry date: Jan 16, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/136
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides tools and methods for the systematic analysis of genetic interactions, including higher order interactions. The present invention provides tools and methods for combinatorial probing of cellular circuits, for dissecting cellular circuitry, for delineating molecular pathways, and/or for identifying relevant targets for therapeutics development.