Method of disturbance correction, and laser scanning microscope having disturbance correction

US12259329B2 · kind B2 · utility

Assignee

• ABBERIOR INSTRUMENTS GMBH · DE

Inventors

• Benjamin Harke · Oettingen, DE
• Roman SCHMIDT · Oettingen, DE
• Lars Kastrup · Göttingen, DE

Key dates

• Filing date: Dec 14, 2020
• Grant date: Mar 25, 2025
• Expiry date: Dec 14, 2041

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/6439
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present disclosure is directed to a method of disturbance correction and to a laser scanning microscope carrying out this method. Specifically, it is directed to an image recording method according to the MINFLUX principle, in which a spatially isolated fluorescence dye molecule is illuminated at a sequence of scan positions by an intensity distribution with a local intensity minimum, and the number of fluorescence photons emitted by the fluorescence dye molecule is detected at each of the scan positions. The location of the molecule is determined with a high spatial resolution from the scan positions and the numbers of fluorescence photons. A disturbance is captured when illuminating the fluorescence dye molecule and detecting the fluorescence light, said disturbance being considered in corrective fashion when determining the location of the fluorescence dye molecule.