Method and device for illuminating a sample in a microscope in points

US12352943B2 · kind B2 · utility

Assignee

• ABBERIOR INSTRUMENTS GMBH · DE

Inventors

• Benjamin Harke · Oettingen, DE
• Lars Kastrup · Göttingen, DE

Key dates

• Filing date: Aug 31, 2021
• Grant date: Jul 8, 2025
• Expiry date: Feb 25, 2044

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B26/0833
• WIPO field: Optics
• WIPO sector: Instruments

Abstract

A method for punctiform illumination of a sample with a MINFLUX microscope has the sample being sequentially illuminated at illumination points of a predefined or predefinable illumination point pattern. The lateral extent of the illumination point pattern is smaller than the longest wavelength of the illumination light. The illumination points are always illuminated exclusively with a time offset. A distinct individual light source is assigned to each illumination point of the illumination point pattern. Each illumination point is illuminated by the focus of an illumination light bundle of the individual light source.