Methods for identifying class 2 crispr-cas systems

US12431216B2 · kind B2 · utility

Assignees

• The Broad Institute, Inc. · US
• Massachusetts Institute of Technology · US
• Rutgers, the State University of New Jersey · US
• The United States of America, as represented by the Secretary, Department of Health and Human Services · US

Inventors

• Sergey Shmakov · Moscow, RU
• Kira S. Makarova · Bethesda, US
• Yuri I. Wolf · Bethesda, US
• Aaron Smargon · Cambridge, US
• Neena Kenton Pyzocha · Cambridge, US
• David Benjamin Turitz Cox · Cambridge, US
• Winston X. Yan · Brookline, US
• David A. Scott · Cambridge, US
• Konstantin Severinov · Highland Park, US
• Feng Zhang · Cambridge, US
• Eugene Koonin · Bethesda, US

Key dates

• Filing date: Aug 16, 2017
• Grant date: Sep 30, 2025
• Expiry date: Jun 30, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G16B15/00
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed here is a method of identifying novel CRISPR effectors, comprising: identifying sequences in a genomic or metagenomic database encoding a CRISPR array; identifying one or more Open Reading Frames (ORFs) in said selected sequences within 10 kb of the CRISPR array; discarding all loci encoding proteins which are assigned to known CRISPR-Cas subtypes and, optionally, all loci encoding a protein of less than 700 amino acids; and identifying putative novel CRISPR effectors, and optionally classifying them based on structure analysis.