Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5210015A · kind A · utility
871Cited by
7References
38Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 6, 1990 |
| Grant date | May 11, 1993 |
| Priority date | — |
| Expiry date | Aug 6, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a process of detecting a target nucleic acid using labeled oligonucleotides. This process uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.