Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5310652A · kind A · utility
215Cited by
4References
7Claims
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Key dates
| Filing date | Jun 24, 1993 |
| Grant date | May 10, 1994 |
| Priority date | — |
| Expiry date | Jun 24, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6876
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.