Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5322770A · kind A · utility
294Cited by
2References
18Claims
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Inventor
Key dates
| Filing date | Dec 22, 1989 |
| Grant date | Jun 21, 1994 |
| Priority date | — |
| Expiry date | Dec 22, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for the replication and amplification of RNA sequences by thermostable DNA polymerases. Reverse transcription of RNA is catalyzed by, for example, 92 kDa Taq, 64 kDa Taq, and Tth DNA polymerase. Reverse transcription is coupled to PCR amplification in a one enzyme procedure using a thermostable polymerase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.