Purified thermostable enzyme
US5352600A · kind A · utility
65Cited by
3References
4Claims
0Family size
Assignee
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Key dates
| Filing date | Nov 5, 1992 |
| Grant date | Oct 4, 1994 |
| Priority date | — |
| Expiry date | Nov 5, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A purified thermostable enzyme is obtained that has unique characteristics. Preferably the enzyme is isolated from the Thermus aquaticus species and has a molecular weight of about 86,000-95,000 daltons. The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. The enzyme is preferably stored in a buffer containing non-ionic detergents that lends stability to the enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.