DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5374553A · kind A · utility
150Cited by
2References
8Claims
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Key dates
| Filing date | Aug 13, 1990 |
| Grant date | Dec 20, 1994 |
| Priority date | — |
| Expiry date | Aug 13, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2521/101
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A purified thermostable enzyme is derived from the eubacterium Thermotoga maritima. The enzyme has a molecular weight of about 97 kilodaltons and DNA polymerase I activity. The enzyme can be produced from native or recombinant host cells and can be used with primers and nucleoside triphosphates in a temperaturecycling chain reaction where at least one nucleic acid sequence is amplified in quantity from an existing sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.