Reduction of non-specific amplification glycosylase using DUTP and DNA uracil
US5418149A · kind A · utility
280Cited by
5References
4Claims
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Key dates
| Filing date | Jan 5, 1993 |
| Grant date | May 23, 1995 |
| Priority date | — |
| Expiry date | Jan 5, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Improved methods for amplifying nucleic acids can reduce non-specific amplification and minimize the effects of contamination of nucleic acid amplification reaction assays due to amplified product from previous amplifications. The methods involve the introduction of unconventional nucleotide bags into the amplification reaction products and treating the products by enzymatic (e.g., glycosylases) and/or physical-chemical means to render the product incapable of acting as a template for subsequent amplifications.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.