Nucleic acid detection by the 5'-3'exonuclease activity of polymerases acting on adjacently hybridized oligonucleotides
US5487972A · kind A · utility
349Cited by
7References
32Claims
0Family size
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Key dates
| Filing date | Jan 5, 1993 |
| Grant date | Jan 30, 1996 |
| Priority date | — |
| Expiry date | Jan 5, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process of detecting a target nucleic acid using labeled oligonucleotides which uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and thus releasing labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.