Purified thermostable nucleic acid polymerase and DNA coding sequences from pyrodictium species
US5491086A · kind A · utility
40Cited by
8References
20Claims
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Key dates
| Filing date | May 14, 1993 |
| Grant date | Feb 13, 1996 |
| Priority date | — |
| Expiry date | May 14, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1252
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Recombinant DNA sequences encoding the DNA polymerase activity of Pyrodictium species can be used to construct recombinant vectors and transformed host cells for production of the activity. Pyrodictium enzymes for catalyzing 3'.fwdarw.5' exonuclease activity, i.e., proofreading enzymes, are also provided. The Pyrodictium enzymes are useful in DNA amplification procedures and are not irreversibly inactivated by exposure to 100.degree. C. in a polymerase chain reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.