Antisense oligonucleotides to the RAS gene

US5582972A · kind A · utility

Assignee

• Isis Pharmaceuticals, Inc. · US

Inventors

• Walter F. Lima · San Diego, US
• Brett P. Monia · Carlsbad, US
• Susan M. Freier · San Diego, US
• David J. Ecker · Encinitas, US

Key dates

• Filing date: Dec 14, 1992
• Grant date: Dec 10, 1996
• Expiry date: Dec 14, 2012

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods are provided for preparing antisense oligonucleotides which take advantage of RNA secondary and tertiary structure and for preparing antisense oligonucleotides which specifically hybridize to regions of RNA secondary and tertiary structure by comparing the affinity of the oligonucleotide for a structured RNA target to the affinity of the oligonucleotide for a length-matched oligonucleotide complement, and selecting an oligonucleotide having an affinity for the structured target which is not less than one thousandth of its affinity for the length-matched oligonucleotide complement. Oligonucleotides are also disclosed which are specifically hybridizable with regions of H-ras RNA having secondary and tertiary structure.