Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5693517A · kind A · utility
63Cited by
5References
6Claims
0Family size
Assignee
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Key dates
| Filing date | Feb 2, 1995 |
| Grant date | Dec 2, 1997 |
| Priority date | — |
| Expiry date | Feb 2, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided. Reagents particularly suited for the methods of the present invention are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.