Reaction mixtures for detection of target nucleic acids
US5804375A · kind A · utility
259Cited by
11References
24Claims
0Family size
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Key dates
| Filing date | Apr 25, 1995 |
| Grant date | Sep 8, 1998 |
| Priority date | — |
| Expiry date | Apr 25, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/815
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.