Method of adenoviral-medicated cell transfection

US5928944A · kind A · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Prem Seth · North Potomac, US
• Ronald G. Crystal · Potomac, US
• Melissa Rosenfeld · Bethesda, US
• Kunihiko Yoshimura · Katsushika, JP

Key dates

• Filing date: Feb 4, 1994
• Grant date: Jul 27, 1999
• Expiry date: Feb 4, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2810/10
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention provides an adenoviral-mediated method of transfection with nucleic acids which can be augmented through incubation of the nucleic acids with cationic agents. Specifically, the present inventive method of introducing a nucleic acid into a eukaryotic cell comprises contacting the cell with, in any order or simultaneously, the nucleic acid and an adenovirus, wherein the nucleic acid is not bound to any molecule capable of effecting its entry into the cell. The cell is preferably additionally contacted with a cationic agent, such as a monocationic or polycationic liposome, such that the nucleic acid is not bound to any molecule capable of effecting its entry into the cell other than, optionally, the cationic agent.