Thermostable DNA polymerases having reduced discrimination against ribo-NTPs
US5939292A · kind A · utility
61Cited by
3References
25Claims
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Key dates
| Filing date | Aug 5, 1997 |
| Grant date | Aug 17, 1999 |
| Priority date | — |
| Expiry date | Aug 5, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides, such as ribonucleotides, into DNA products, are advantageous in many in vitro synthesis applications. Such enzymes are particularly useful for use in nucleic acid sequencing protocols and provide novel means for DNA sequence analysis. Genes encoding the modified enzymes and methods for their production and use offer cost and efficiency advantages for DNA sequencing.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.