Kits for amplifying and detecting nucleic acid sequences, including a probe
US6040166A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 27, 1994 |
| Grant date | Mar 21, 2000 |
| Priority date | — |
| Expiry date | Sep 27, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S977/92
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a process for amplifying any target nucleic acid sequence contained in a nucleic acid or mixture thereof using a thermostable enzyme. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers with a thermostable enzyme to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction can be repeated as often as desired and involve temperature cycling to effect hybridization, promotion of activity of the enzyme, and denaturation of the hybrids formed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.